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Studying is a skill and it is an art. Being successful in school requires a high level of study skills. Students must first learn these skills, practice them and develop effectively study practice in order to be successful. Very often the study practice and practices developed and used are not permanent throughout the student life.Good study practices include many different skills: time management, self discipline, concentration, memorization, organization, and effort. Without good study practice, a student cannot succeed. To succeed, students must able to appropriately assimilate course content, digest it, reflect on it, and be able to articulate that information in written and/or oral form. It is the ability to acquire effective study skills. Many people feel the hours of study are the most important.Scientific aptitude plays a major role in understanding the contents of science subject. It is a special intellectual ability to comprehend scientific facts and knowledge .It is a specific ability which enables the individual to acquire scientific knowledge and understanding through the process of teaching and learning. While teaching any lesson of science subject, particularlyPhysics, Chemistry and Biology at the Higher Secondary level in the class room.A teacher introduces meaningful and interesting science related activities which arouse thescientific temper and understanding among Higher Secondary Science students. The students are able to grasp in their mind about the science concept, because of the role of Study Practices and Scientific aptitude makes the students to be able to understand,apply,analyse, organize , synthesize,evaluate and create the scientific concepts in a meaningful and purposeful way. Once the students learn with healthy study practices thoroughly the science subject, it makes them easy to perform well in the term examinations. The purpose of this study is to analyse the study practices and scientific aptitude in higher secondary science students. The study adopted a descriptive method with normativesurvey technique. A sample of 215 higher secondary plus one students was selectedinTrichirappalli District as sample for this study by using a stratified random sample technique. The data was analysed by using descriptive,differential and correlation analysis. The study found a significant and positive relationshipbetween study practices and scientific aptitude of Higher Secondary Science students.Furthere, the study found that the higher secondary boys and girls do not differ significantly in respect of their Study Practices and Scientific Aptitude.